quickblocktm blocking buffer for immunol staining (beyotime) (Beyotime)

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Beyotime quickblocktm blocking buffer for immunol staining (beyotime)
Quickblocktm Blocking Buffer For Immunol Staining (Beyotime), supplied by Beyotime, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/quickblocktm+blocking+buffer+(beyotime)/quickblocktm+blocking+buffer+for+immunol+staining/pmc12276682-76-29-31
Average 90 stars, based on 1 article reviews

quickblocktm blocking buffer for immunol staining (beyotime) - by Bioz Stars, 2026-09

90/100 stars

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Western Blot:

Article Title: Deliver CEBPE via cartilage targeting Lipid nanoparticle to block CEBPE-LTF-STAT3 positive feedback loop for efficient treatment of cartilage endplate degeneration
Article Snippet: Denatured proteins were separated using SDS-PAGE and transferred onto PVDF membranes (Millipore, USA). .. The membranes were blocked with QuickBlockTM Western blocking buffer (Beyotime, Cat. No: P0252) at room temperature for 30 min, followed by overnight incubation at 4 °C with the following primary antibodies: CEBPE (1:1000, Santa Cruz, sc-515192), Collagen II (1:1000, Proteintech, 28459-1-AP), MMP13 (1:1000, Proteintech, 18165-1-AP), RUNX2 (1:1000, Abcam, ab236639), OPN (1:1000, Abcam, ab166709), IL-1β (1:1000, Proteintech, 26048-1-AP), TNF (1:1000, Proteintech, 17590-1-AP), JAK2 (1:1000, Abcam, ab108596), P-JAK2 (1:1000, Abcam, ab32101), STAT3 (1:1000, Abcam, ab68153), P-STAT3 (1:1000, Abcam, ab267373), α-Tubulin (1:10000, Proteintech, 11224-1-AP), and GAPDH (1:50000, Proteintech, 60004-1-Ig). .. Following incubation, the membranes underwent three washes with TBST buffer, then were incubated with the respective secondary antibodies at ambient temperature for a duration of 2 h. Detection of chemiluminescence was accomplished using an advanced chemiluminescent reagent (UElandy, Product Code: S6009M), and the resultant signals were captured using the Bio-Rad ChemiDoc Touch imaging system.

Article Title: Milk-derived exosomes exert anti-inflammatory activity in lipopolysaccharide-induced RAW264.7 cells by modulating the TLR4/NF-κB and PI3K/AKT signaling pathways
Article Snippet: Proteins were subsequently transferred from the gel to a PVDF membrane (MilliporeSigma). .. The PVDF membrane was treated with a blocking solution (QuickBlockTM Western; cat. no. P0252; Beyotime Institute of Biotechnology) to inhibit non-specific binding at room temperature for 10 min, then the membranes were incubated at 4 ̊C for 12-16 h with the primary antibodies. ..

Blocking Assay:

Article Title: Exosomal miR-30a-5p promoted intrahepatic cholangiocarcinoma progression by increasing angiogenesis and vascular permeability dependent on PDCD10
Article Snippet: .. Then, the gel was transferred to nitrocellulose membranes, blocked with QuickBlockTM Blocking Buffer (Beyotime, China) for 15 minutes, incubated with primary antibody overnight at 4◦C and with secondary antibody at room temperature for 2h. .. The exposed Proteins images were visualized with ECL Plus (EMD Millipore, Billarica, MA, USA).

Article Title: Decoding hepatobiliary-specific immune gene patterns in gastrointestinal cancers via gene ontology fingerprints, multi-omics, and experimental integration
Article Snippet: .. After incubating the sections in 3% H 2 O 2 for 20 min, they were blocked with Beyotime QuickBlockTM blocking buffer (P0260) for 30 min. .. Subsequently, the TMA was incubated with the primary antibody (MIF, STARTER, S0B0858) overnight at 4°C, followed by incubation with a Texas Red-X conjugated secondary antibody (Invitrogen, T-2767) for 1 h. Each step was followed by three 5-min washes with PBS.

Article Title: Rewiring of 3D chromatin topology orchestrates transcriptional reprogramming in muscle fiber-type specification and transformation.
Article Snippet: .. After three washes, they were blocked in QuickBlockTM Blocking Buffer (Beyotime, P0220) for 2 h, followed by incubation with the primary antibody overnight and the secondary antibody for 1 h. Nuclei were counterstained with DAPI. .. Multiplex immunofluorescence staining based on tyramide signal amplification was performed by using four-color multiple fluorescent immunohistochemical staining kit (Absin, abs50012) according to the manufacturer’s protocols.

Article Title: Rewiring of 3D chromatin topology orchestrates transcriptional reprogramming in muscle fiber-type specification and transformation
Article Snippet: All protein levels were normalized to corresponding β-tubulin or GAPDH, and densitometric quantification of the bands was performed using ImageJ software. .. Cells were fixed with 4% paraformaldehyde and permeabilized using 0.5% Triton X-100 for 30 min. After three washes, they were blocked in QuickBlockTM Blocking Buffer (Beyotime, P0220) for 2 h, followed by incubation with the primary antibody overnight and the secondary antibody for 1 h. Nuclei were counterstained with DAPI. .. Multiplex immunofluorescence staining based on tyramide signal amplification was performed by using four-color multiple fluorescent immunohistochemical staining kit (Absin, abs50012) according to the manufacturer’s protocols.

Article Title: Novel TCF21high pericyte subpopulation promotes colorectal cancer metastasis
Article Snippet: .. After that, tumor sections were permeabilized in 287 0.1% TritonTM X-100, blocked with QuickBlockTM immunostaining blocking solution 288 (Cat. ST797, Beyotime, Shanghai, China) and incubated with the corresponding 289 primary antibody overnight at 4 °C. ..

Incubation:

Immunostaining:

Membrane:

Binding Assay:

Immunofluorescence:

Article Title: Supporting Information
Article Snippet: .. After 3 days, cells were fixed in PFA for 15 min and incubated with quickblockTM blocking buffer (Beyotime, China) for immunofluorescence staining for 15 min. Primary antibody was added and incubated overnight, including COL I, Scleraxis (Scx), and tenomodulin (Tnmd). .. Subsequently, the appropriate secondary antibodies (Cy3-AffiniPure Goat Anti-Rabbit IgG (H+L) or Cy3-AffiniPure Goat AntiMouse IgG (H+L)) were added.

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